DOK2 pTyr299 Rabbit Polyclonal Antibody
CAT#: AP02521PU-S
DOK2 pTyr299 rabbit polyclonal antibody, Aff - Purified
Size: 100 ul
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CNY 1,400.00
货期*
2周
规格
Specifications
Product Data | |
Applications | IF, IHC, WB |
Recommend Dilution | Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100. |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human p56Dok-2 around the phosphorylation site of tyrosine 299 (G-E-YP-A-V). |
Specificity | This antibody detects endogenous levels of p56Dok-2 only when phosphorylated at Tyrosine 299. |
Formulation | PBS(without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol State: Aff - Purified State: Liquid purified Ig fraction. |
Concentration | lot specific |
Purification | Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site. |
Conjugation | Unconjugated |
Storage Condition | Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
Gene Name | docking protein 2 |
Database Link | |
Background | Docking proteins interact with receptor tyrosine kinases and mediate particular biological responses using signal transduction. DOK2 acts as a multiple docking protein downstream of receptor or non-receptor tyrosine kinases. By this mechanism it acts to negatively regulate signal transduction and cell proliferation controlled by cytokines in a feedback loop. DOK2 is highly expressed in cells and tissues of hematopoietic origin as well as in lung. Expression of bcr/abl induces additional tyrosine phosphorylation of the DOK1 and DOK2 proteins and their association with Ras-GAP. Thus, it is suspected that DOK association regulates GAP activity toward Ras and that the DOK proteins serve as mediators of bcr-abl signaling. The role of DOK proteins in bcr-abl regulation may also be implicated in chronic myelogenous leukemia (CML), which is characterized by a Philadelphia chromosome translocation t(9;22). Such a mutation would result in a p210-bcr/abl chimeric protein-tyrosine kinase which has been found in many CML cases. |
Synonyms | p56(dok-2), p56Dok-2, Docking protein 2 |
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