MEK2 (MAP2K2) pThr394 Rabbit Polyclonal Antibody

CAT#: AP02310PU-N

MEK2 (MAP2K2) pThr394 rabbit polyclonal antibody, Aff - Purified

Size: 50 ul 100 ul



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CNY 2,250.00


货期*
2周

规格
    • 100 ul

Product images

Specifications

Product Data
Applications IF, IHC, WB
Recommend Dilution Western blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
Immunohistochemistry on paraffin sections: 1/50 - 1/100.
Immunofluorescence: 1/100 - 1/200.
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human MEK2 around the phosphorylation site of threonine 394 (P-G-TP-P-T).
Specificity MEK2 (phospho-Thr394) antibody detects endogenous levels of MEK2 only when phosphorylated at threonine 394.
Formulation Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl containing 0.02% Sodium Azide as preservative and 50% glycerol as stabilizer
State: Aff - Purified
State: Liquid purified Ig fraction
Concentration lot specific
Purification Immunoaffinity chromatography
Conjugation Unconjugated
Storage Condition Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Predicted Protein Size 44 kDa
Gene Name mitogen-activated protein kinase kinase 2
Background Microtubule associated protein kinase kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK 1 & 2/MAPK enzymes by phosphorylating both residues within the threonine/glutamic acid/tyrosine (TEY) motif in the activation loop. MEK 1 & 2 are also activated by dual phosphorylation, which occurs on serines 218 and 222, in the activation loop of the MEKs. Threonine 394 of MEK2 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK2.
Synonyms MAP kinase kinase 2, ERK activator kinase 2, MAPK/ERK kinase 2, MEK2, MKK2, MAP kinase kinase 2
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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