Collagen XVII (COL17A1) (507-548) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommend Dilution	ELISA: 1/5000. Western blot: 1/500 (Ref.2)  Immunocytochemistry (Ref.3).  Immunocytochemistry on Frozen Sections: 1/100 (Ref.3).
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Glutathione-S-transferase fusion protein containing the sequence of the immunodominant region of human type XVII collagen / BP180 (aa 507-548)
Specificity	This antibody detects Human type XVII Collagen / BP180 (aa 507-548).
Formulation	50mM Tris, pH 7.4 State: Ig Fraction State: Lyophilized purified IgG fraction
Reconstitution Method	Restore in aqua bidest to a concentration of 1 mg/ml.
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C and reconstituted at -20°C. Avoid repeated freezing and thawing.
Gene Name	collagen type XVII alpha 1
Database Link	Entrez Gene 1308 Human UniProt ID Q9UMD9
Background	Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in the gene coding for collagen XVII are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD 1, is generated by proteolytic processing of the full length form. Two transcript variants, one resulting from alternative splicing in the 3' UTR, have been identified for this gene.
Synonyms	COL17A1, BP180, BPAG2, Bullous pemphigoid antigen 2, LAD-1, LABD97
Reference Data
Protein Families	Transmembrane

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