SPARC Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: 1/5,000 (See also Ref.1). Western Blot: 1/2,000 (See also Ref.2,3). Immunohistochemistry on Paraffin Sections: 1/1,000.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant Human Osteonectin
Specificity	This antibody detects Osteonectin. There were no cross reactivities obtained with Human Osteopontin, and Human Bone Sialoprotein.
Formulation	State: Serum State: Lyophilized Serum
Reconstitution Method	Restore in aqua bidest to initial volume.
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	secreted protein acidic and cysteine rich
Database Link	Entrez Gene 6678 Human UniProt ID P09486
Background	SPARC (secreted protein acidic and rich in cysteine)/Osteonectin is a matricellular glycoprotein that modulates cellular interactions with the ECM and is expressed in tissues undergoing remodeling. It functions as a de-adhesive protein, as a modulator of growth factor activity, and as a cell-cycle inhibitor. It induces changes in endothelial cell shape via F-actin, coincident with the appearance of intercellular gaps, that provide a paracellular pathway for extravasation of macromolecules. Tumor growth is enhanced in mice lacking SPARC due to changes in the ECM that create a more permissive environment for tumor progression.
Synonyms	ON, Basement-membrane protein 40, BM-40
Note	GeneID 6678
Reference Data

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