p53 (TP53) pSer9 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western Blot: 1/500-1/1000. Immunohistochemistry on paraffin sections: 1/50-1/200.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Specificity	This antibody detects endogenous levels of p53 when phosphorylated at Ser9.
Formulation	Phosphate buffered saline (PBS), pH~7.2 containing 0.05% Sodium Azide as preservative. State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 48 kDa
Gene Name	tumor protein p53
Database Link	Entrez Gene 7157 Human UniProt ID P04637
Background	The p53 protein was initially identified in a complex with the SV40 large T antigen in SV40-transformed rodent cells. Subsequently, p53 was shown to form complexes with adeno-virus E1B protein and the E6 protein encoded by oncogenic papillomavirus variants. Although p53 was initially considered to be a cellular proto-oncogene, it has since been classified as a tumor suppressor gene or anti-oncogene. Presumably, the presence of excess amounts of the mutant form of the protein can lead to functional inactivation of wild type p53. Mutations and allelic loss of the p53 gene have been associated with malignant transformation in a wide variety of human tumors. These findings in combination with the fact that p53 can act as a negative regulator of cell growth in normal cells suggest that the binding of p53 by various DNA tumor virus-encoded transforming proteins may be a major contributing factor to their transforming phenotype.
Synonyms	Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.