MEK2 (MAP2K2) pThr394 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, IP, WB
Recommend Dilution	ELISA: 1/10000-1/20000. Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200. Immunoprecipitation: 1/50-1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Specificity	This antibody detects endogenous levels of MEK2 pThr394 protein.
Formulation	Phosphate buffered saline (PBS), pH~7.2 containing 15 mM Sodium Azide as preservative. State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	mitogen-activated protein kinase kinase 2
Database Link	Entrez Gene 5605 Human UniProt ID P36507
Background	Cell proliferation and development are carefully controlled in C. elegans, with each cell following a nearly invariant pattern of differentiation. Vulval development in particular provides a useful model for studying how cell fate is determined. In addition to cell signaling pathways such as Notch and Ras pathways, the establishment of cell polarity and the asymmetric distribution of certain receptors are also critical for proper cell fate determination. In C. elegans, activated Ras initiates a cascade of sequential phosphorylation events, including the protein kinases Raf, MEK, and MAP kinase. The LET-60 Ras-mediated signal transduction pathway controls vulval induction. LIN-45, a member of the Raf family of serine/threonine kinases, and LIN-31, an HNF-3 homologe, act downstream of the Ras protein as necessary components for vulval differentiation. LIN-1 contains an ETS domain and is thought to be a substrate for the ERK subfamily of MAP kinases. The C. elegans proteins MEK-2, a MAP kinase kinase, and SUR-2 also function in the LET-60 Rasmediated vulval induction.
Synonyms	MAP kinase kinase 2, ERK activator kinase 2, MAPK/ERK kinase 2, MEK2, MKK2, MAP kinase kinase 2
Reference Data

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