CDK2 pThr160 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western Blot: 1/500-1/1000. Immunohistochemistry on paraffin sections: 1/50-1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Specificity	p-CDK2 (pThr160) antibody detects endogenous levels of p-CDK2 protein.
Formulation	Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. State: Aff - Purified State: Liquid purified Ig fraction
Concentration	1.0 mg/ml
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 34 kDa
Gene Name	cyclin-dependent kinase 2
Database Link	Entrez Gene 1017 Human UniProt ID P24941
Background	Cell cycle progression is dependent on the sequential activation of cyclindependent kinases (Cdks). For full activity, the cell cycle control protein Cdk2 requires phosphorylation of a conserved residue, Threonine 160, carried out by Cdk-activating kinase 1. The kinase associated phosphatase (KAP) is a human dual specificity protein phosphatase that dephosphorylates Cdk2 on Threonine 160 in a cyclin dependent manner. KAP binds to Cdk2 and dephosphorylates Threonine 160 when the associated cyclin subunit is degraded or dissociates. Fluorescence measurements show that Threonine 160 phosphorylation increases the affinity of Cdk2 for both histone substrates and ATP and decreases its affinity for ADP.
Synonyms	p33 protein kinase
Reference Data

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