beta 2 Adrenergic Receptor (ADRB2) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western Blot: 1/500 - 1/1000. Immunohistochemistry on paraffin sections: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 351-400 of Human AR-β2.
Specificity	This antibody detects endogenous levels of AR-beta2 protein. (region surrounding Cys378)
Formulation	Phosphate buffered saline (PBS), pH~7.2 State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE). Preservative: 0.05% Sodium Azide
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen.
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 47 kDa, 68 kDa (glycosylated), 95 kDa (Dimer)
Gene Name	adrenoceptor beta 2
Database Link	Entrez Gene 154 Human UniProt ID P07550
Background	Adrenergic receptors (ARs) (the term ?adrenergic? reflects the alternative name for epinephrine, adrenaline) include four general types (alpha1, alpha2, beta1 and beta2) which are found in different target tissues and differ in their affinities and responses to various agonists and antagonists. cDNA clones have been isolated for all of the major AR subtypes and a number of closely related receptors have been identified by this approach. Each of the receptors have been shown to consist of single polypeptide chains which transverse the plasma membrane seven times, presumably forming a bundle of helices within the membrane. These transmembrane regions are hydrophobic and are interconnected by extracellular and intracellular hydrophilic loops. The coupling of ARs to specific intracellular effectors is mediated through diverse heterotrimeric G proteins and is regulated by G protein-coupled receptor kinases (GRKs), cAMP-dependent protein kinase A and protein kinase C directed phosphorylation.
Synonyms	ADRB2, ADRB2R, B2AR, Beta-2 adrenoreceptor
Reference Data

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