MIA2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA: Direct: To detect hMIA-2 (using 100 μl/well antibody solution) a concentration of 0.25 - 1.0 μg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIA-2. Sandwich: To detect hMIA-2 (using 100 μl/well antibody solution) a concentration of 0.25 - 1.0 μg/ml of this antibody is required. In conjunction with Polyclonal Anti-Human MIA-2 as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIA-2. Western blot: To detect hMIA-2 this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIA-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (> 98 %) recombinant human MIA-2
Specificity	This antibody detects MIA-2.
Formulation	PBS, pH 7.2 Label: Biotin State: Sterile filtered lyophilized Ig fraction
Reconstitution Method	Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 -1 .0 mg/ml.
Purification	Affinity chromatography
Conjugation	Biotin
Storage Condition	Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C. Avoid repeated freezing and thawing.
Gene Name	CTAGE family member 5
Database Link	Entrez Gene 4253 Human UniProt ID Q96PC5
Background	MIA2 (Melanoma inhibitory activity 2) is a secreted cytokine and a member of the MIA/OTOR family. It is predominantly expressed in hepatocytes and may play a role in the pathophysiology of liver disease. Elevated levels of MIA2 may represent a clinically useful marker for diagnosis of hepatic disease activity and severity. MIA2 may act as a tumor suppressor in hepatocellular carcinoma.
Synonyms	Melanoma inhibitory activity protein 2
Reference Data

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