Melanoma Inhibitory Activity (MIA) Rabbit Polyclonal Antibody
CAT#: AP01143BT-S
Melanoma Inhibitory Activity (MIA) rabbit polyclonal antibody, Biotin
Size: 50 ug
Conjugation: Unconjugated
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CNY 3,910.00
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Specifications
Product Data | |
Applications | ELISA, WB |
Recommend Dilution | Direct ELISA: To detect Human MIA (using 100 μl/well antibody solution) a concentration of ~1.0 μg/ml of this antibody is required. It allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIA. Sandwich ELISA: To detect Human MIA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. In conjunction with Polyclonal Anti-Human MIA (AP01143PU-S or AP01143PU-N) as a Capture antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIA. Western blot: To detect Human MIA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human MIA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Highly pure (> 98%) recombinant Human MIA |
Specificity | This antibody detects MIA. |
Formulation | PBS, pH 7.2 without preservatives. Label: Biotin State: Lyophilized (sterile filtered) purified Ig fraction |
Reconstitution Method | Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1 - 1.0 mg/ml. |
Purification | Affinity Chromatography. |
Conjugation | Biotin |
Storage Condition | Store the lyophilized antibody at -20°C. Following reconstitution it is stable for two weeks at 2-8°C. Frozen aliquots are stable for 6 months when stored at -20°C. Avoid repeated freezing and thawing. |
Gene Name | melanoma inhibitory activity |
Database Link | |
Background | Melanoma Inhibitory Activity (MIA) was originally identified as an inhibitor of the in vitro growth of malignant melanoma cells. It was the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family; Melanoma Inhibitory Activity, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3)-like domain. Melanoma Inhibitory Activity is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extracellular matrix in vivo. Elevated levels of Melanoma Inhibitory Activity may represent a clinically useful marker for diagnosis of melanoma metastasis as well as a potential marker for rheumatoid arthritis. |
Synonyms | Melanoma inhibitory activity |
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