Apolipoprotein E (APOE) (Isoform E3) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Indirect ELISA:  To detect hApoE3 (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hApoE3. Sandwich ELISA: To detect hApoE3 (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. In conjunction with Biotinylated Anti-Human ApoE3 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hApoE3. Western blot: To detect hApoE3 this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hApoE3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (> 98%) E.coli derived, 34.4 kDa recombinant human ApoE3
Specificity	This antibody detects ApoE3.
Formulation	PBS, pH 7.2 without preservatives. State: Aff - Purified State: Sterile filtered lyophilized Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1 - 1.0 mg/ml.
Purification	Immunoaffinity chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	apolipoprotein E
Database Link	Entrez Gene 348 Human UniProt ID P02649
Background	Apolipoprotein E is essential for the normal catabolism of triglyceride rich lipoprotein constituents. The apolipoprotein E gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in Apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Mutations in the APOE gene confer susceptibility to Alzheimer's disease by affecting amyloid-beta deposition.
Synonyms	ApoE, Apo-E
Note	Centrifuge vial prior to opening.
Reference Data

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