Apolipoprotein E (APOE) (Isoform E3) Rabbit Polyclonal Antibody
CAT#: AP01142BT-N
Apolipoprotein E (APOE) (Isoform E3) rabbit polyclonal antibody, Biotin
Size: 25 ug
Conjugation: Unconjugated
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CNY 4,790.00
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Specifications
Product Data | |
Applications | ELISA, WB |
Recommend Dilution | Direct ELISA: To detect Human ApoE3 by Direct ELISA (using 100 µl/well antibody solution) a concentration of ~1.0 µg/ml of this antibody is required. This Biotinylated polyclonal antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human ApoE3. Sandwich ELISA: To detect Human ApoE3 by Sandwich ELISA (using 100 µl/well) a concentration of 0.25-1.0 µg/ml of this antibody is required. This Biotinylated antibody, in conjunction with Affinity purified anti-Human ApoE3 (AP01142PU-N or AP01142PU-S) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human ApoE3. Western blot: To detect Human ApoE3 this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human ApoE3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | E.Coli derived recombinant Human ApoE3. |
Specificity | This antibody detects ApoE3. Cross reacts with Human ApoE2 and Human ApoE4 in both Sandwich ELISA and Western Blot. |
Formulation | PBS, pH 7.2 without preservatives. Label: Biotin State: Lyophilized (sterile filtered) purified Ig fraction. |
Reconstitution Method | Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. |
Purification | Affinity Chromatography. |
Conjugation | Biotin |
Storage Condition | Store the lyophilized antibody at -20°C. Following reconstitution it is stable for two weeks at 2-8°C. Frozen aliquots are stable for 6 months when stored at -20°C. Avoid repeated freezing and thawing. |
Gene Name | apolipoprotein E |
Database Link | |
Background | Apolipoprotein E3 mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo E receptor (chylomicron remnant) of hepatic tissues. |
Synonyms | ApoE, Apo-E |
Note | Centrifuge vial prior to opening. Molecular Weight: ~150 kDa |
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