PLGF (PGF) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Sandwich ELISA: To detect Human PlGF (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. In conjunction with Biotinylated Anti-Human PlGF as a detection antibody (Cat.-No AP01135BT), it allows the detection of at least 0.2-0.4 ng/well of recombinant Human PlGF. Western blot: To detect Human PlGF this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human PlGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.  Immunohistochemistry on Paraffin Sections: This antibody stained formalin-fixed, paraffin-embedded sections of Human breast invasive ductal carcinoma. Recommended Concentration: 2.0 μg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended (See Figures).
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (> 98%) E.coli derived from recombinant Human PlGF
Specificity	This antibody detects Human PIGF. Other species not tested.
Formulation	PBS, pH 7.2 State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/ml.
Purification	Immunoaffinity Chromatography employing immobilized Human PlGF matrix
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	placental growth factor
Database Link	Entrez Gene 5228 Human UniProt ID P49763
Background	PLGF is a growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration. It belongs to the cysteine-knot superfamily. PLGF is expressed in placental tissues, colon and mammary carcinomas. It signals through the VEGFR-1/FLT1 receptor.
Synonyms	PGFL, PLGF, PlGF
Reference Data

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