CD40L (CD40LG) Goat Polyclonal Antibody
CAT#: AP01120PU-N
CD40L (CD40LG) goat polyclonal antibody, Aff - Purified
Size: 50 ug
Conjugation: Biotin
Need it in bulk or conjugated?
Get a free quote
CNY 4,790.00
Product images
![](https://cdn.origene.com/img/defaults-img.jpg)
Specifications
Product Data | |
Applications | ELISA, IHC, WB |
Recommend Dilution | Sandwich ELISA: To detect Human sCD40 Ligand (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml is required. In conjunction with Biotinylated Anti-Human sCD40-Ligand (Cat.-No AP01120BT-N or AP01120BT-S) as a detection antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human sCD40-Ligand. Western Blot: To detect Human sCD40-Ligand by Western blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human sCD40-Ligand is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry on Paraffin sections: 2.5-5.0 µg/ml, Overnight at 4°C. This antibody stained sections of human cervical squamous cell carcinoma. An HRP-labeled polymer detection system was used with a DAB Chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium citrate buffer is recommended. |
Reactivity | Human |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Highly pure (> 98%) E.coli derived recombinant Human sCD40L Cat.-No PA151 |
Specificity | This antibody detects Human sCD40 Ligand / CD154. |
Formulation | PBS, pH 7.2 State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction |
Reconstitution Method | Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/ml. |
Purification | Immunoaffinity Chromatography employing immobilized Human sCD40 Ligand matrix |
Conjugation | Unconjugated |
Storage Condition | Prior to reconstitution store at 2-8°C. |
Predicted Protein Size | ~ 150 kDa |
Gene Name | CD40 ligand |
Database Link | |
Background | CD40 ligand (CD40L) is a 33 kDa type II membrane glycoprotein expressed mainly on the cell surface of activated T lymphocytes, but also exists as a soluble form extracellularly. CD40L is the ligand for CD40, a member of the TNF superfamily, which is expressed on the cell surface of B cells, macrophages/monocytes, dendritic cells, vascular endothelial cells, and epithelial cells. CD40L plays an important role in B cell proliferation, antibody class switching, modulation of apoptosis in the germinal center through interaction with B cells expressing CD40, and activation of CD4+ T cells. Mutation within the CD40L gene is linked to hyper IgM syndrome, an X linked immunodeficiency disease that is characterized by elevated level of serum IgM and decreased level of other isotypes. |
Synonyms | CD40 ligand, CD40-L, CD40LG, TNFSF5, TRAP, GP39 |
Reference Data |
Documents
Product Manuals |
FAQs |
SDS |
Resources
抗体相关资料 |