Persephin (PSPN) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Indirect ELISA: Indirect: To detect hPersephin by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPersephin. Sandwich ELISA: To detect hPersephin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml is required. In conjunction with Biotinylated Anti-Human Persephin as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPersephin. Western Blot: To detect hPersephin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPersephin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (> 98%) E.coli derived 20.8 kDa recombinant human Persephin
Specificity	This antibody detects Persephin.
Formulation	PBS, pH 7.2 without preservatives. State: Aff - Purified State: Sterile filtered lyophilized Ig fraction
Reconstitution Method	Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/ml.
Purification	Immunoaffinity purified
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	persephin
Database Link	Entrez Gene 5623 Human UniProt ID O60542
Synonyms	PSPN, PSP
Reference Data

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