BMP7 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect BMP-7 by Direct ELISA (using 100 μl/well antibody solution) a concentration of ~1.0 μg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human BMP-7. Sandwich ELISA: To detect BMP-7 by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. In conjunction with Polyclonal Anti-Human BMP-7 (Cat.-No AP01113PU) as a capture antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human BMP-7. Western Blot: To detect BMP-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human BMP-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) E.coli derived recombinant Human BMP-7 (Cat.-No PA1006)
Specificity	This antibody detects Human BMP7. Other species not tested.
Formulation	PBS, pH 7.2 Label: Biotin State: Lyophilized (Sterile filtered) purified Ig fraction
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Centrifuge vial prior to opening.
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	bone morphogenetic protein 7
Database Link	Entrez Gene 655 Human UniProt ID P18075
Background	Bone Morphogenetic Protein 7 (BMP7), also known as osteogenic protein 1 (OP1), is produced from a DNA sequence encoding the human BMP2 signal peptide and human BMP2 propeptide (amino acid residues 1 to 282) fused to the human BMP7 mature subunit (amino acid residues 293 to 431). Mature human BMP7, generated after the proteolytic removal of the signal peptide and the propeptide, is a disulfide linked homodimeric protein, comprised of two 139 amino acid residue subunits. Bone Morphogenetic Proteins (BMP) are members of the TGF beta superfamily of cytokines that affect bone and cartilage formation. Similar to other TGF beta family proteins, BMPs are highly conserved across animal species.
Synonyms	BMP-7, Bone morphogenetic protein 7, OP1, Osteogenic protein 1
Reference Data

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