Lgi1 (37-113) Mouse Monoclonal Antibody [Clone ID: S283-7]

Specifications

Product Data
Clone Name	S283-7
Applications	IF, IHC, WB
Recommend Dilution	Western blot: 1/1000; 1μg/ml was sufficient for detection of LGI1 / EPT in 20 μg of rat brain lysate by colorimetric immunoblot using HRP conjugated secondary antibody. Immunocytochemistry. Immunohistochemistr: Free floating sections, fixed in formaldehyde.
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Fusion protein amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse LGI1 /EPT, SwissProt: Q9JIA1.1.
Specificity	This antibody detects a 60 kDa protein corresponding to the molecular weight of LGI1 / EPT. It will recognize only the longer form, Isoform 1. (~50% identity with LGI2, LGI3 and LGI4.)
Formulation	PBS pH 7.4, 50% Glycerol, 0,09% Sodium azide State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Protein G chromatography
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	leucine-rich repeat LGI family, member 1
Database Link	Entrez Gene 56839 Mouse UniProt ID Q9JIA1
Background	The leucine-rich, glioma inactivated gene 1 (LGI1) was first identified as a candidate tumor suppressor gene for glioma and may play a role in other cancers. LGI1 is a member of a family of highly related proteins containing leucine-rich repeats (LRRs) which are highly similar to other transmembrane signaling molecules and receptors. LGI1 serves as a ligand to ADAM22, a metalloprotease localized at the synapse. Mutations in LGI1 account for nearly half of autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms. Two isoforms of LGI1 are known to exist.
Synonyms	Epitempin-1
Reference Data

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