VEGF Receptor 2 (KDR) Mouse Monoclonal Antibody [Clone ID: KDR721]

Specifications

Product Data
Clone Name	KDR721
Applications	FC, IF, IHC, IP
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Flow Cytometry: 0.5-1 µg/million cells.  Immunofluorescence: 0.5-1 µg/ml.  Functional Studies: Use BSA and Azide Antibody. Immunoprecipitation: 0.5-1 µg/ 500 µg protein lysate. Immunohistochemistry on Frozen Sections: 0.5-1.0 µg/ml for 30 minutes at RT. Positive Control: HUVEC cells, Human lymph nodes and tonsils.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human VEGF-R2 protein.
Specificity	Recignizes Human VEGF-R2 / CD309 / Flk-1 / KDR3. Other species not tested.  Cellular Localization: Cell surface.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	150-230 kDa
Gene Name	kinase insert domain receptor
Database Link	Entrez Gene 3791 Human UniProt ID P35968
Background	CD309, also known as VEGF-R2, KDR3, and Flk-1 (mouse), is a type I transmembrane glycoprotein. It is a member of the CSF-1/PDGF receptor family of type III tyrosine kinase receptors. Human VEGF-R2 is mainly expressed by endothelial cells, embryonic tissues, and megakaryocytes. It plays an important role in the regulation of angiogenesis, vasculogenesis, and vascular permeability. The ligands of VEGF-R2 include VEGF-A, VEGF-C, VEGF-D, and VEGF splice isoforms. Ligation of VEGF-R2 with its ligands results in the receptor dimerization and auto-phosphorylation, stimulating endothelial cell proliferation and migration.
Synonyms	VEGFR2, FLK1, KDR, VEGF Receptor 2
Reference Data

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