EGFR Mouse Monoclonal Antibody [Clone ID: H9B4]

Specifications

Product Data
Clone Name	H9B4
Applications	IP, WB
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Immunoprecipitation: 1-2 µg/500ug protein lysate. Western Blotting: 0.5-1.0 µg/ml for 2 hours at RT. Positive Control: A431 cells, Breast or Bladder cancer.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified EGFR from A431 cells.
Specificity	This MAb reacts with a cytoplasmic domain of EGFR. EGFR is a type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin & epiregulin. Cellular Localization: Cell surface.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	~170 kDa (wild type) and ~145 kDa (vIII variant)
Gene Name	epidermal growth factor receptor
Database Link	Entrez Gene 1956 Human UniProt ID P00533
Background	EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
Synonyms	Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Reference Data

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