EGFR Mouse Monoclonal Antibody [Clone ID: B1D8]

Specifications

Product Data
Clone Name	B1D8
Applications	FC, IP
Recommend Dilution	Affinity Purification (For coupling, order Antibody without BSA). Blocks Ligand-induced Activation of EGFR.  Inhibits Proliferation of A431 Cells (Order Antibody without Sodium Azide). Arrests Tumor Growth in vivo (Order Ab without BSA and Azide).  Immunoprecipitation: 1-2 µg/500 µg protein lysate.  Flow Cytometry: 0.5-1 µg/million cells. Positive Control: A431 cells, Breast or Bladder cancer
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Microsomes from A431 cells.
Specificity	This antibody reacts with the extracellular domain of EGFR and blocks the EGF/TGFα−induced activation. It also blocks tumor growth in vivo. It is excellent for purification of EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. Cellular Localization: Cell surface.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	~170 kDa (wild type) and ~145 kDa (vIII variant)
Gene Name	epidermal growth factor receptor
Database Link	Entrez Gene 1956 Human UniProt ID P00533
Background	EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
Synonyms	Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Reference Data

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