Integrin beta 4 (ITGB4) Mouse Monoclonal Antibody [Clone ID: UM-A9]

Specifications

Product Data
Clone Name	UM-A9
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	Biological Blockade: Partially blocks laminin binding (Use Azide free Antibody).  ELISA: Use BSA free Antibody for coating. Flow Cytometry: 0.5-1 µg/million cells.  Immunofluorescence: 0.5-1 µg/ml.  Western Blotting (not tested): 0.5-1 µg/ml/million cells in 0.1 ml.  Immunoprecipitation (not tested): 0.5-1 µg/500 µg protein lysate. Immunohistochenmistry on Frozen Sections (not tested): 0.5-1 µg/ml for 30 minutes at RT. Positive Control: A431 cells, squamous cell carcinoma, colon or placenta.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human squamous cell carcinoma (UM-SCC1).
Specificity	Recognizes a protein of 205kDa, which is identified as integrin beta-4 (ITGB4). Its epitope is localized in the extracellular domain of ITGB4 protein. Integrins are heterodimers comprised of alpha and beta subunits, that are non-covalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta-4 subunit, a receptor for the laminins. This subunit tends to associate with alpha-6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Cellular Localization: Cell surface.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	205 kDa
Gene Name	integrin subunit beta 4
Database Link	Entrez Gene 3691 Human UniProt ID P16144
Synonyms	ITGB4, GP150
Reference Data

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