CD34 Mouse Monoclonal Antibody [Clone ID: SPM123]

Specifications

Product Data
Clone Name	SPM123
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	Flow Cytometry: 0.5-1 µg/million cells.  Immunofluorescence: 0.5-1 µg/ml.  Immunoprecipitation: 0.5-1 µg/500 µg protein.  Western Blotting: 0.25-0.5 µg/ml.  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: KG-1 cells, Tonsil, or Angiosarcoma.
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Detergent solubilized vesicular suspension prepared from human term placenta.
Specificity	This Monoclonal Antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified into three main categories, class I, class II and class III. It is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain. CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi’s sarcoma, but with a lower specificity. Cellular Localization: Cell surface.  Negative Species:  Rat, Sheep, Cow and Dog.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	90-110 kDa
Gene Name	CD34 molecule
Database Link	Entrez Gene 947 Human UniProt ID P28906
Synonyms	Hematopoietic progenitor cell marker
Reference Data

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