TTF1 (NKX2-1) Mouse Monoclonal Antibody [Clone ID: SPM150]

Specifications

Product Data
Clone Name	SPM150
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Flow Cytometry: 0.5-1 µg/million cells.  Immunofluorescence: 0.5-1 µg/ml. Western Blotting: 0.5-1 µg/ml.  Immunoprecipitation: 1-2 µg/500 µg protein lysate.  Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 µg/ml for 30 minutes at RT.  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: MAD109, MLE-15, H441-4, or H345 cells. Normal thyroid or lung.
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat full length TTF-1 recombinant protein.
Specificity	Recognizes a protein of 40kDa, identified as Thyroid transcription factor-1 (TTF-1). TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies. Cellular Localization: Nuclear.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	40 kDa
Gene Name	NK2 homeobox 1
Database Link	Entrez Gene 7080 Human UniProt ID P43699
Synonyms	NKX2-1, Nkx-2.1, TITF1, Thyroid nuclear factor 1, Thyroid transcription factor 1, TTF-1
Reference Data

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