alpha smooth muscle Actin (ACTA2) Mouse Monoclonal Antibody [Clone ID: SPM332]

CAT#: AM50166PU-S

alpha smooth muscle Actin (ACTA2) mouse monoclonal antibody, clone SPM332, Purified

Size: 20 ug 100 ug



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CNY 5,606.00


货期*
5周

规格
    • 100 ug

Cited in 1 publication.

Product images

Specifications

Product Data
Clone Name SPM332
Applications FC, IF, IHC, IP, WB
Recommend Dilution Flow Cytometry: 0.5-1 μg/million cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blotting: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistology (Formalin-Fixed): 0.5-1.0 μg/ml for 30 minutes at RT.
(Staining of Formalin-Fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min.)
Positive Control: Blood vessels in all tissues, smooth muscle or leiomyosarcoma.
Reactivity Bovine, Canine, Chicken, Feline, Guinea Pig, Goat, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Baboon
Host Mouse
Clonality Monoclonal
Immunogen N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH.
Specificity Actin is a major component of the cytoskeleton and is present in most cell types.
This Monoclonal Antibody is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This  Monoclonal Antibody does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
Cellular Localization: Cytoplasmic.
Formulation 10mM PBS
State: Purified
State: Liquid purified IgG fraction from Bioreactor Concentrate
Stabilizer: 0.05% BSA
Preservative: 0.05% Sodium Azide
Concentration lot specific
Purification Protein A/G Chromatography
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C.
Predicted Protein Size 42 kDa
Gene Name actin, alpha 2, smooth muscle, aorta
Synonyms ACTSA, ACTVS, Alpha-actin-2
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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