CD45 (PTPRC) (CD45RO) Mouse Monoclonal Antibody [Clone ID: SPM125]

Specifications

Product Data
Clone Name	SPM125
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Flow Cytometry: 0.5-1 μg/million cells. Immunofluorescence: 1-2 μg/ml. Western Blotting: 0.5-1 μg/ml. Immunoprecipitation: 1-2 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1.0 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: CCRF-CEM, Jurkat or MOLT-4 cells. Paracortex in a tonsil or a reactive lymph node.
Reactivity	Chimpanzee, Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Cultured human T-cells from an IL-2-dependent T-cell line (CA1).
Specificity	Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas. Cellular Localization: Cell surface.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	180-185 kDa
Gene Name	protein tyrosine phosphatase, receptor type C
Database Link	Entrez Gene 5788 Human UniProt ID P08575
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data

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