FOXP3 Mouse Monoclonal Antibody [Clone ID: SPM579]

Specifications

Product Data
Clone Name	SPM579
Applications	FC, IF, IHC
Recommend Dilution	Flow Cytometry: 0.5-1 μg/million cells. Immunofluorescence: 0.5-1 μg/ml. Immunohistochemistry on Formalin-Fixed Paraffin Sections: 0.5-1.0 μg/ml for 30 minutes at RT. (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min. followed by cooling at RT for 20 minutes). Positive Control: Tonsil, lymph node or Breast carcinoma.
Reactivity	Human, Monkey, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Full-length human FOXP3 protein.
Specificity	Recognizes a protein of 47-55kDa, which is identified as FOXP3. Its precise epitope is not known, but it has been mapped to the N-terminal portion of the protein.  Cellular Localization: Predominantly nuclear, some cytoplasmic.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	47-55 kDa
Gene Name	forkhead box P3
Database Link	Entrez Gene 50943 Human UniProt ID Q9BZS1
Background	The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. During early development, FOXP1 and FOXP2 are expressed abundantly in the lung, with lower levels of expression in neural, intestinal and cardiovascular tissues, where they act as transcription repressors. FOXP1 is widely expressed in adult tissues, while neoplastic cells often exhibit a dramatic change in expression level or localization of FOXP1. Mutations in FOXP3 gene cause IPEX, a fatal, X-linked inherited disorder characterized by immune dysregulation. The FOXP3 protein is essential for normal immune homeostasis. Specifically, FOXP3 represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation.
Synonyms	Forkhead box protein P3, IPEX, JM2, Scurfin
Reference Data

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