MUC2 Mouse Monoclonal Antibody [Clone ID: SPM296]
CAT#: AM50142PU-T
MUC2 mouse monoclonal antibody, clone SPM296, Purified
Size: 100 ug
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CNY 3,223.00
货期*
5周
规格
Specifications
Product Data | |
Clone Name | SPM296 |
Applications | FC, IF, IHC, IP, WB |
Recommend Dilution | ELISA: Use BSA free Antibody for coating. Flow Cytometry: 0.5-1 μg/million cells. Immunofluorescence: 1-2 μg/ml. Western Blotting: 0.5-1 μg/ml. Immunoprecipitation: 1-2 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-fixed Sections: 0.5-1.0 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: LS174T cells or small intestine. |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | A synthetic peptide of 29 amino acids from MUC2 protein, coupled to KLH. |
Specificity | Recognizes a single glycoprotein of 520kDa, identified as mucin 2 (MUC2). This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Mucins are high molecular weight glycoproteins, which constitute the major component of the mucus layer that protects the gastric epithelium. MUC2 is specifically expressed in goblet cells of the small intestine & colon; in about 65% of colonic carcinomas and about 40% of gastric carcinomas. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary. Cellular Localization: Cytoplasmic and cell surface. |
Formulation | 10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide |
Concentration | lot specific |
Purification | Protein A/G Chromatography |
Conjugation | Unconjugated |
Storage Condition | Store undiluted at 2-8°C. |
Predicted Protein Size | 520 kDa |
Gene Name | mucin 2, oligomeric mucus/gel-forming |
Database Link | |
Synonyms | MUC-2, MUC2, SMUC, Intestinal mucin-2 |
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