Cytokeratin 10 (KRT10) Mouse Monoclonal Antibody [Clone ID: SPM261]

Specifications

Product Data
Clone Name	SPM261
Applications	IF, IHC, IP, WB
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Flow Cytometry: 0.5-1 µg/million cells. Immunofluorescence: 0.5-1 µg/ml. Western Blotting: 0.25-0.5 µg/ml. Immunoprecipitation: 0.5-1 µg/500 µg protein lysate. Immunohistochemistry on Frozen and Formalin-fixed Sections: 0.5-1.0 µg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8-9, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: A431, HeLa, MCF7 cells or Esophagus.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Skin extract of a Human Psoriasis patient.
Specificity	This MAb recognizes a protein of 56.5kDa, identified as Cytokeratin 10 (CK10). Cellular Localization: Cytoplasmic.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	56.5 kDa
Gene Name	keratin 10
Database Link	Entrez Gene 3858 Human UniProt ID P13645
Background	CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
Synonyms	Cytokeratin-10, CK10, CK-10, Keratin-10, KRT10, KPP, K10
Reference Data

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