MALT1 Mouse Monoclonal Antibody [Clone ID: SPM578]

Specifications

Product Data
Clone Name	SPM578
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 1-2 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 1-2 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: Jurkat, Daudi or HeLa cells, or lymphoma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human fragment of MALT1 protein (701-808).
Specificity	Recognizes Human MALT1/Paracaspase. Other species not tested. Cellular Localization: Cytoplasmic.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	93 kDa
Gene Name	MALT1 paracaspase
Database Link	Entrez Gene 10892 Human UniProt ID Q9UDY8
Background	MALT1/Paracaspase (MALT1) was independently identified as a member of the human paracaspase family and an interacting partner of B-cell lymphoma (Uren et al., 2000). MALT1 is a caspase-like protein that contains an N-terminal death domain, two Ig-like domains, and a C-terminal caspase-like domain. It binds to Bcl-10 through its Ig-like domains and cooperates with Bcl-10 to activate NF-kappaB. MALT1 is thought to play an important role in NF-kappaB signaling by enchancing NF-kappaB activation through interaction with Bcl-10 (Ho et al., 2005). Interaction between MALT1 and Bcl-10 mediates IKK activation in vitro through facilitatiing the ubiquitination of NEMO by the ubiquitinconjugating enzyme UBC13.
Synonyms	Mucosa-associated lymphoid tissue lymphoma translocation protein 1, MLT, Paracaspase
Reference Data

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