GCLM Mouse Monoclonal Antibody [Clone ID: AT2D12]

Specifications

Product Data
Clone Name	AT2D12
Applications	ELISA, FC, IF, WB
Recommend Dilution	ELISA. Western blot: 1/3000 (using HeLa cells (40 µg), resolved by SDS-PAGE). Flow cytometry: 2-5 µg in 1x106 cells (in HeLa cell line). Immunofluorescence/Immnunocytochemistry: 1/100 (in HeLa cell line).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human GCLM (1-274aa) purified from E. coli
Formulation	PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein-G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	glutamate-cysteine ligase modifier subunit
Database Link	Entrez Gene 2730 Human UniProt ID P48507
Background	GCLM (Glutamate-cysteine ligase, modifier subunit), also known as gamma-glutamylcysteine synthetase, is first rate limiting enzyme of glutathione synthesis. GCLM is heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic antioxidants, heat shock, oxidants and cytokines. GCLM deficiency has been implicated in some forms of hemolytic anemia. Also, GCLM is a molecular target for amelioration of cisplatin restance in lung cancer.
Synonyms	Gamma-ECS regulatory subunit, GCS light chain
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Glutathione metabolism, Metabolic pathways

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