CD8A Mouse Monoclonal Antibody [Clone ID: C8/468 + C8/144B]

Specifications

Product Data
Clone Name	C8/468 + C8/144B
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	Flow Cytometry: 0.5-1 µg/106 cells. Immunofluorescence: 0.5-1 µg/ml. Western Blot (Not tested): 0.5-1 µg/ml. Immunoprecipitation (Not tested): 0.5-1 µg/500 µg protein lysate. Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml. Immunohistochemistry on Paraffin Sections: 0.5-1 µg/ml. Incubation Time: 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: HuT78 or hPBL, Tonsil.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human CD8 recombinant protein (C8/468); A 13 amino acid syntheti c peptide from the C-terminal cytoplasmic domain of alpha chain of human CD8 molecule (C 8/144B).
Specificity	Recognizes Human CD8. Other species not tested. Cellular Localization: Cell surface.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	32 kDa
Gene Name	CD8a molecule
Database Link	Entrez Gene 925 Human UniProt ID P01732
Background	CD8 is a cell surface glycoprotein molecule expressed on the surface of a subset of T cells, generally the cytotoxic/suppressor cells, as well as on some natural killer cells. There are two forms of CD8, either as an alpha-alpha homodimer or alpha-beta heterodimer. The pathological role of CD8 has been observed in familial CD8 deficiency. A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.
Synonyms	CD8 alpha chain, CD8A, MAL
Reference Data

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