L1CAM Mouse Monoclonal Antibody [Clone ID: UJ127]

Specifications

Product Data
Clone Name	UJ127
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Flow Cytometry: 0.5-1 μg/million cells. Immunofluorescence: 1-2 μg/ml. Western Blotting: 0.5-1 μg/ml. Immunoprecipitation: 1-2 μg/500 μg protein lysate. Immunohistochemistry on Frozen Sections: 1-2 μg/ml for 30 minutes at RT. Positive Control: Neuroblastomas or Schwannomas.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Homogenous suspension of 16 week Human fetal brain.
Specificity	Recognizes a cell surface protein of 220-240kDa, identified as L1 Cell Adhesion Molecule. This Monoclonal Antibody is useful in the identification of primitive neuroectodermal tumors. It binds to tumors of neuroectodermal and glial origin e.g. neuroblastoma and Schwannomas. It does not bind to pediatric or adult brain. Cellular Localization: Cell surface.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	220-240 kDa
Gene Name	L1 cell adhesion molecule
Database Link	Entrez Gene 3897 Human UniProt ID P32004
Background	The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS (hydrocephalus-stenosis of the aqueduct of Sylvius); 2) MASA (mental retardation, aphasia, shuffling gait, and adducted thumbs); and 3) SPG1 (spastic paraplegia). The L1, neural cell adhesion molecule (L1CAM) also plays an important role in axon growth, fasciculation, and neural migration as well as in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm.
Synonyms	N-CAM L1, CAML1, MIC5
Reference Data

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