TRP1 (TYRP1) Mouse Monoclonal Antibody [Clone ID: TA99]

Specifications

Product Data
Clone Name	TA99
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Flow Cytometry: 0.5-1 µg/million cells. Immunofluorescence: 1-2 µg/ml. Western Blotting (Not tested): 0.5-1 µg/ml. Immunoprecipitation: 1-2 µg/500 µg protein lysate. Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT. Positive Control: SK-MEL-23, SK-MEL-19, SK-MEL-30, SK-MEL-37 cells or Melanoma.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	SK-MEL-23 cells.
Specificity	It reacts with a 75kDa melanocyte-specific gene product, identified as Tyrosinase-related protein-1 (TRP-1). Cellular Localization: Cytoplasmic.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	75 kDa
Gene Name	tyrosinase related protein 1
Database Link	Entrez Gene 7306 Human UniProt ID P17643
Background	Tyrosinase-related protein-1 (TRP-1) is involved in melanin synthesis. TRP1 is present on the melanosomal membranes of melanoma, normal melanocytes and nevi. Recent evidence suggests that TRP-1 is involved in maintaining stability of tyrosinase protein and modulating its catalytic activity. TRP-1 is also involved in maintenance of melanosome ultrastructure and affects melanocyte proliferation and cell death. Defects in TRP-1 affect pigmentation in melanosomes, and have been shown to cause two diseases dependent on skin colour; rufous oculocutaneous albinism (ROCA) occurs predominantly in southern African blacks and oculocutaneous albinism type III (OCA-III/3) a form of albinism with only moderate reduction of pigment.
Synonyms	Tyrosinase-related protein 1, TYRP, TYRRP, TRP-1, TRP1, DHICA oxidase, Catalase B, CAS2
Reference Data

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