TGF alpha (TGFA) Mouse Monoclonal Antibody [Clone ID: MF9]

Specifications

Product Data
Clone Name	MF9
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use free BSA Antibody for coaating.  Western Blotting: 0.5-1.0 µg/ml. Flow Cytometry: 0.5-1 µg/million cells.  Immunofluorescence: 1-2 µg/ml. Immunoprecipitation: 1-2 µg/500 µg protein lysate.  Immunohistochemistry on Frozen Sections: 1-2 µg/ml for 30 minutes at RT. Positive Control: Jurkat or Ramos cells, Heart, Kidney, Pituitary, Breast cancer.
Reactivity	Bovine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	A 17-amino acid synthetic peptide from the carboxyl-terminus of Rat TGF-alpha.
Specificity	This antibody reacts with the C-terminus of TGF alpha and sho ws no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Antibody is completely blocked by the peptide used for raising this antibody. Cellular Localization: Cytoplasmic and secreted.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	~6 kDa
Gene Name	transforming growth factor alpha
Database Link	Entrez Gene 7039 Human UniProt ID P01135
Background	TGF-alpha (aa 50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
Synonyms	Transforming growth factor alpha, ETGF
Reference Data

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