MHC Class I Heavy Chain (Restricted expression) Mouse Monoclonal Antibody [Clone ID: HCA2]

Specifications

Product Data
Clone Name	HCA2
Applications	ELISA, FC, IF, IHC, IP, WB
Recommend Dilution	ELISA. Western Blot. Immunoprecipitation. Flow Cytometry. Immunocytochemistry. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections (See References for Conditions). Recommended Dilutions: 1/100–1/200 for Flow Cytometry, and for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1/100–1/1000 for Immunoblotting applications.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from BALB/c mice immunized with HLA-B7 and -B40 heavy chains.
Specificity	This Monoclonal antibody HCA2 recognize HLA class I heavy chains. The reactivity spectrum of HCA2 is composed of all HLA-A chains (except HLA-A24), as well as some HLA-B, HLA-C, HLA-E, HLA-F, and HLA-G chains. The antibody HCA2 reacts preferentially with HLA-A locus heavy chains. HCA2 was raised against free class I heavy chains of HLA, to obtain antibodies that would still react with denatured class I antigens, as they occur in Western blotting, conventional light microscopical analysis of Formalin-Fixed, Paraffin-Embedded Sections, and Cryo-Immuno-Electron Microscopy. HCA2 indeed retains strong reactivity with free class I heavy chains in Western blots. HCA2 in particular reacts in a locus-specific manner by biochemical criteria. HCA2 also produces strong reactivity in Immuno-Electron Microscopy. Its use allows the determination of tissue and subcellular distribution of class I antigens.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	The HLA class I gene family is composed of a group of genes whose products encode cell surface glycoproteins of MW 40–45 kDa, associated non-covalently with the beta-2-microglobulin light chain. They include the three polymorphic molecules HLA-A, -B, and -C, which are ubiquitously expressed and which are able to present intracellular peptides to cytotoxic T cells. Three additional class I genes are known, commonly referred to as non-classical or class Ib genes, all highly homologous to the other class I genes and all of which associate with beta-2-microglobulin light chain. In humans, each of the class Ib genes appears to exhibit a distinct pattern of expression in developing and adult tissues. HLA-E transcripts are distributed widely in adult tissues and have also been found in the placenta and fetal liver. In the adult, the presence of HLA-F has been shown in skin, resting T cells, and B cells, whereas its expression during development has been reported in fetal liver and at low levels in placenta and extra-placental tissues. HLA-G was originally thought to be expressed only in certain populations of placental trophoblasts, but low levels have also been found in a variety of human tissues. Recently it was shown that HLA class I expression in breast cancer cells can have a predictive value for chemotherapy response.
Synonyms	HLA Class 1, MHC Class 1, Major Histocompatibility complex class I
Reference Data

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