CD11a (ITGAL) (Activitor) Mouse Monoclonal Antibody [Clone ID: NKI(SPV)-L16]

Specifications

Product Data
Clone Name	NKI(SPV)-L16
Applications	FC, IP
Recommend Dilution	Flow Cytometry. Immunoprecipitation.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Immunization of Balb/c mice with monocytes. The unique activation dependent epitope of the SPV-L16 antibody on the a-chain of LFA-1 is relatively insensitive to trypsin treatment and thus most likely located close to the transmembrane segment of the protein.
Specificity	This antibody recognizes an activation epitope of CD11a and has been essential to define inside-out signaling of intergrin. Homotypic cell aggregate formation is stimulated. A unique antibody NKI(SPV)-L16, reacting with the a-chain of the human leukocyte function-associated Ag-1 (LFA-1) has been made available. This antibody stimulates homotypic cell-cell interactions in a manner very similar to 12-0-tetradecanoyl-phorbol1-3-acetate (TPA), in contrast to other anti-LFA-1 mAb which inhibit cell aggregation. Immunoprecipitation and enzyme digestion studies revealed that NKI-L16 recognizes a unique epitope on the cy-chain of LFA-1, most likely situated close to the transmembrane segment of the molecule. It is hypothesized that NKI-L16 or TPA can cause the LFA-1 molecule to convert from an inactive to an active configuration, thereby permitting binding of LFA-1 1 to its natural ligand. This mAb directed, in contrast to other anti-LFA-1 antibodies, stimulates rather than inhibits homotypic cell aggregate formation, but does not affect cell proliferation.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles.
Gene Name	integrin subunit alpha L
Database Link	Entrez Gene 3683 Human UniProt ID P20701
Background	Integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide), also known as ITGAL, is a human gene which functions in the immune system. It is involved in cellular adhesion and costimulatory signaling. ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 is part of the family of leukocyte integrins that are recognised by their common β-chains (CD18). LFA-1 also has a distinct α-chain (CD11a). LFA-1 is expressed on lymphocytes, monocytes and granulocytes; it has been shown that LFA-1 contributes to the adhesion reaction of these cells. Blocking experiments with anti-LFA monoclonal antibodies demonstrated that LFA-1 inhibits the adhesion step effector and target cells in cytotoxic T lymphocyte, natural killer and lectin dependent cytotoxicity. LFA-1 is involved in recruitment to the site of infection. It binds to ICAM-1 on antigen-presenting cells and functions as an adhesion molecule. LFA-1 is the first to bind T-cells to antigen-presenting cells and initially binds weakly. A signal from the T-cell receptor and/or the cytokine receptor changes the conformation and prolongs the cell contact, allowing the T-cell to proliferate.
Synonyms	Integrin alpha-L, LFA1, LFA-1
Reference Data

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