Collagen IV (COL4A1) Mouse Monoclonal Antibody [Clone ID: 1043]

Specifications

Product Data
Clone Name	1043
Applications	FC, IF, IHC, WB
Recommend Dilution	Western blot: 1/100-1/1000. Immunocytochemistry on fixed cells (methanol fixation). Flow cytometry. Immunohistochemistry on Frozen Sections using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. Immunohistochemistry on Paraffin Sections: Pretreatement with 1 mg/ml pepsin in 0.01N HCl for 30 minutes at room temperature is recommended. Recommended Dilutions: 1/50–1/100 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified Human type IV Collagen, isolated from Human placenta.  Antibody derived by fusion of Sp 2/0 mouse myeloma cells with spleen cells from a BALB/c mouse.
Specificity	This antibody clone 1043 recognizes type IV collagen in the extracellular – basement membranes of normal and pathological tissues.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles.
Gene Name	collagen type IV alpha 1 chain
Database Link	Entrez Gene 1282 Human UniProt ID P02462
Background	Type IV collagen is an integral part of most, if not all basement membranes. The extent of expression of the basement membrane component type IV collagen may be correlated with the degree of differentiation of basement membrane producing neoplasms. Neoplastic cells of invasive carcinomas often lack a continuous basement membrane. In diagnostic histopathology antibodies to type IV collagen may help to distinguish between non-invasive (benign or in situ) and invasive lesions. Furthermore the antiserum can be of help in the differential diagnosis of bullous lesions in dermatopathology.
Synonyms	COL4A1
Reference Data

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