Vimentin (VIM) Mouse Monoclonal Antibody [Clone ID: 2D1]

Specifications

Product Data
Clone Name	2D1
Applications	IF, WB
Recommend Dilution	Western Blot: 1/1000.  Immunofluorescence:  1/500.
Reactivity	Bovine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human vimentin purified from E. coli.
Specificity	Specific for the ~50kDa Vimentin protein.
Formulation	PBS State: Aff - Purified State: Liquid purified IgG fraction Preservative: 10mM Sodium Azide
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	vimentin
Database Link	Entrez Gene 7431 Human UniProt ID P08670
Background	Vimentin is the major protein subunit of the 10nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration (Nieminen M et al. 2006). Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness (Zhao et al. 2008).
Synonyms	VIM
Reference Data

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