Cd55 Rat Monoclonal Antibody [Clone ID: 3D5]

Specifications

Product Data
Clone Name	3D5
Applications	FC, WB
Recommend Dilution	Flow Cytometry. Western blot.  The typical starting working dilution is 1/50.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	NRK cells expressing transmembrane-anchored Mouse DAF
Specificity	The monoclonal antibody 3D5 recognizes complement decay accelerating factor (DAF), also designated as CD55.
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	CD55 molecule, decay accelerating factor for complement
Database Link	Entrez Gene 13136 Mouse UniProt ID Q61475
Background	Cells express on their surface several proteins which protect against complement attack, namely C receptor I (CR1), decay accelerating factor (DAF), membrane cofactor protein (MCP) and CD59. CR1, DAF and MCP regulate the activation pathways of complement by either accelerating decay of the C3 and C5 convertase (CR1, DAF), or acting as cofactors for the serine protease factor I, which cleaves and irreversibly inactivates C3b (CR1, MCP). Mouse DAF (CD55) is a 60 kDa transmembrane protein that binds C3b and C4b to inhibit formation and half-life of the C3 convertases. DAF is broadly distributed among cells in contact with serum, including both haematopoietic and nonhaematopoietic cells. Although DAF does not have an essential role in controlling hemolysis of erythrocytes, it has an important role in regulation of the deposition of C3 on nucleated cells. Together with other complement regulators DAF protects self cells from autologous complementmediated injury. DAF cooperates with CD46 in circumventing autologous C3 deposition, while CD59 inhibits the pathway at the critical end-point.
Synonyms	CR; CROM; DAF; TC
Reference Data

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