CD45 (PTPRC) Mouse Monoclonal Antibody [Clone ID: 2B11]

Specifications

Product Data
Clone Name	2B11
Applications	IF, IHC, IP, WB
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: Ramos, U-698, or GA-10 cells, Tonsil.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Isolated neoplastic cells from T cell lymphoma.
Specificity	This Monoclonal SPM570 antibody recognizes Human CD45. Other species not tested. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. Cellular Localization: Cell surface and cytoplasmic.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	180-220 kDa
Gene Name	protein tyrosine phosphatase, receptor type C
Database Link	Entrez Gene 5788 Human UniProt ID P08575
Background	CD45, a transmembrane multifunctional glycoprotein, is a member of the Type I receptorlinked PTPase family. Its physiological functions include T and Bcell activation and proliferation, negative regulation of T and Bcell antigen receptor signaling and cytokinereceptor signaling, negative regulation of IL-3 mediated cellular proliferation, EPOdependant homeopoiesis and anti-viral responses, regulation of integrin-mediated adhesion and migration of immune cells, chemokine-induced T-cell chemotaxis, MHC-II signaling, IgE mediated degranulation in mast cells, CD40L-induced microglial activation and IL-4 mediated IgE class switch recombination in Bcells. Loss of CD45 has been implicated in SCID, Alzheimer's disease and multiple sclerosis. CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase.
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data

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