HLA-DRA Mouse Monoclonal Antibody [Clone ID: IPO-10]

Specifications

Product Data
Clone Name	IPO-10
Applications	FC, IF, IHC
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Flow Cytometry: 0.5-1 µg/106 cells. Immunofluorescence: 0.5-1 µg/ml. Positive Control: Ramos, Daudi or HuT78 cells, Tonsil or lymph node.
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Spleen cells of a patient with hairy cell leukemia (Daudi cells).
Specificity	MAb IPO-10 defines the antigen, which appears on B cell progenitors following HLA-DR and preceding CD10, CD19, CD22, CD37 and cym. It is expressed on resting B cells and than reappears and persists in cytoplasm and on cell surface until cytoplasmic Ig appears. It is a useful antibody for diagnostics of neoplasms of B cell origins. It reacts with human B cell lines Daudi, Raji, Namalva, EB-3, RPMI-8226 (50% of cells). The MAb does not label T cell lines, blood granulocytes, thymocytes or bone marrow stromal fibroblasts. No significant changes are detected after PHA or ConA stimulation while LPS and PWM stimulated cultures after 18-48h show decreased number of antigen-positive cells but in final terms of cultivation antigen is expressed again. This MAb labels B cell leukemias and some lymphomas. Hairy cell leukemia strongly reacts and 70% of B cell CLL and some B-NHL were also positive. IPO-10 reacts with AMML cells and in a majority of Hodgkins disease cases a significant percentage of affected lymph node cells were detected. Cellular Localization: Cell surface.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	~36 kDa
Gene Name	major histocompatibility complex, class II, DR alpha
Database Link	Entrez Gene 3122 Human UniProt ID P01903
Background	HLA DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of an alpha chain (36 kDa) and a beta chain (27 kDa). It is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T helper cells. They therefore have a critical role in the initiation of the immune response.
Synonyms	HLA-DRA1, MHC class II antigen DRA
Reference Data

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