CD95 (FAS) Mouse Monoclonal Antibody [Clone ID: B-R18]

Specifications

Product Data
Clone Name	B-R18
Recommend Dilution	Flow Cytometry.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	BALB/c mice (Iffa Credo) were immunized with purified recombinant Fas antigen and fused with X63/Ag.8653 mouse myeloma cells.
Specificity	This antibody clone B-R18 specifically recognizes CD95. B-R18 reacts with peripheral lymphocytes and especially with peripheral monocytes. It stains human B cell lines like pre-B cells, EBV cells, Burkitt cells and plasmacytoma cells. It also binds to human T cell lines, myeloid cell lines, hepatocyte carcinoma and endothelial cells.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.05% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	Fas cell surface death receptor
Database Link	Entrez Gene 355 Human UniProt ID P25445
Background	CD95, also known as FAS or APO1, is a 36 kDa cell surface type I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS PAGE. CD95 is a member of the TNF receptor family, which includes TNFR1, TNFR2, CD27, CD30 and CD40. Binding of CD95 Ligand to CD95 or crosslinking of CD95 by anti CD95 monoclonal antibodies leads to apoptosis of CD95 expressing cells. CD95 belongs to a subgroup of family members that have a death domain (DD) which contains 70 amino acids near the carboxyl terminal region of the molecule. The binding of adaptor molecules to this DD is responsible for transmitting the death signal for apoptosis. Stimulation of CD95 results in aggregation of its DD, leading to the recruitment of FADD and caspase 8 that together with the receptor form the death inducing signaling complex (DISC). CD95/CD95L is involved in the peripheral deletion of activated mature T cells at the end of the immune response and defects in this pathway predispose to autoimmune disorders. CD95 is also involved in killing of targets such as virus infected cells or cancer cells and killing of inflammatory cells at immune privileged sites.
Synonyms	FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6
Reference Data

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