Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody [Clone ID: M9]

Specifications

Product Data
Clone Name	M9
Applications	IF, IHC, WB
Recommend Dilution	Immunoblotting. Immunofluorescence. Immunohistochemistry on Frozen Sections: ~1/150, preferably in PBS. Immunohistochemistry on Paraffin Sections: A positive result on paraffin embedded tissues could be obtained with a TUF pretreatment at a antibody dilution of 1/10.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Cytokeratin isolated from Human breast carcinoma cell line MCF-7.
Specificity	In Immunoblotting experiments the antibody clone M9 recognizes only CytoKeratin 18 (45 kD) and lacks cross reactivity with other Cytokeratins. The antibody stains especially simple epithelia. Cross reactivity GFAP : not detectable Neurofilament : not detectable Vimentin : not detectable Desmin : not detectable
Formulation	State: Supernatant State: Liquid Tissue Culture Supernatant Preservative: Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	keratin 18
Database Link	Entrez Gene 3875 Human UniProt ID P05783
Background	Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.
Synonyms	Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18
Reference Data

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