c Kit (KIT) (C-term) Rabbit Monoclonal Antibody [Clone ID: YR145]

Specifications

Product Data
Clone Name	YR145
Applications	IHC
Recommend Dilution	Immunohistochemistry on frozen and paraffin sections: 1/25 - 1/100. Preparation and Pretreatment:  1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C.  2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water.  3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step. Positive control: Breast, GIST, Skin, Testes Staining pattern: Cytoplasmic, Membranous
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Specificity	This antibody reacts to CD117.
Formulation	PBS, pH 7.4 State: Supernatant State: Liquid supernatant Stabilizer: 0,9% BSA Preservative: 0,09% sodium azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	KIT proto-oncogene receptor tyrosine kinase
Database Link	Entrez Gene 3815 Human UniProt ID P10721
Background	CD117, c-kit is a tyrosine kinase receptor found on interstitial cells of Cajal, germ cells, bone marrow stem cells, melanocytes, breast epithelium and mast cells. This receptor is found on a wide variety of tumor cells (follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, breast; malignant melanoma, endodermal sinus tumor, and small cell carcinoma) but has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi’s sarcoma, and tumors of smooth muscle origin.
Synonyms	SCFR, KIT
Reference Data

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