Alkaline Phosphatase (ALPP) Mouse Monoclonal Antibody [Clone ID: R92F6]

Specifications

Product Data
Clone Name	R92F6
Applications	IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections.  Immunohistochemistry on Paraffin Sections. Recommended Dilution: 1/50-1/100.  Positive Control: Infected tissue. Staining Pattern: Cytoplasmic, Nuclear.  Preparation and Pretreatment: 1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C.  2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water.  3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	Anti-Parvovirus antibody targets the capsid proteins VP1 and VP2 on Human Parvovirus. Parvovirus B19 infection has been implicated as a cause in spontaneous abortion in humans and thus application of this antibody to placental tissues in such cases is appropriate. Parvovirus B19 is also associated with erythema infectiosum (fifth disease) in children and acute arthritis in adults, as well as chronic hemolytic anemia, with some patients experiencing aplastic crisis.
Formulation	PBS, 7.4 State: Supernatant State: Liquid diluted Supernatant Stabilizer: 0.9% BSA Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.  Avoid repeated freezing and thawing.
Gene Name	alkaline phosphatase, placental
Database Link	Entrez Gene 250 Human UniProt ID P05187
Synonyms	PLAP-1, Alkaline phosphatase Regan isozyme
Reference Data

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