Human Lambda Light Chain Mouse Monoclonal Antibody [Clone ID: 48]

Specifications

Product Data
Clone Name	48
Applications	ELISA, IHC
Recommend Dilution	ELISA. Immunohistochemistry on Frozen Sections: Use at 1/100 dilution preferably in PBS. Immunohistochemistry on Paraffin Sections: Use at 1/10 dilution. A positive result on Paraffin Embedded tissue could be obtained with a TUF pretreatment.  Recommended Positive Control: Tonsil.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	In ELISA the antibody will react with free Lambda chains and also with Lambda chains attached to heavy chains. In Ouchterlony Immunodiffusion, no reaction with Bence Jones Lambda or Ig Lambda. No crossreactivity with Kappa chains.  The antibody stains Lambda chain containing cells in sections of sublimate Fixed and Paraffin Embedded tissues. Membrane bound Ig containing Lambda Light chains will also be recognized as well as cytoplasmic Ig from bone marrow cells containing Lambda chains. All cells reacting with conventional anti-Lambda will also react with this antibody.
Formulation	PBS State: Supernatant State: Liquid Supernatant Preservative: 10 mM Sodium Azide, 1% FCS
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Background	All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).
Reference Data

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