Cd8a Mouse Monoclonal Antibody [Clone ID: R73-2b]

Specifications

Product Data
Clone Name	R73-2b
Applications	IHC
Recommend Dilution	Flow Cytometry. Immunohistochemistry on Fresh-Frozen Tissues. It can also be applied in assays that are based on T cell activation.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Specificity	The R73-2b Monoclonal antibody binds to an epitope of the constant region of the Rat alpha/beta-T cell receptor and is able to activate Rat T cells (Beun et al., 1993). The reactivity of R73-2b Monoclonal antibody is Rat strain-independent. R73-coated culture flasks induce Rat T cell proliferation (Beun et al., 1993). This Monoclonal antibody is an IgG2b isotype switch variant (Beun et al., 1993) of the well-known R73 IgG1 Monoclonal antibody that is specific for the Rat alpha/beta-T cell receptor (Hünig et al. 1989).
Formulation	State: Supernatant State: Hybridoma Culture Supernatant Preservative: 0.05% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for Two months or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD8a molecule
Database Link	Entrez Gene 24930 Rat UniProt ID P07725
Background	The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. TCR is a heterodimer composed of either α and β or γ and δ chains. The vast majority of circulating T cells (95%) express the α/β heterodimer while roughly 2-5% express the γ/δ heterodimer. CD3 chains and the CD4 or CD8 coreceptors are also required for efficient signal transduction through the TCR. The TCR is expressed on T helper and T cytotoxic cells that can be distinguished by their expression of CD4 and CD8. T helper cells express CD4 proteins and T cytotoxic cells display CD8. CD4 is also expressed on cortical cells, mature medullary thymocytes, microglial cells and dendritic cells. CD4, also designated T4 and Leu 3, is a 55 kDa membrane glycoprotein that contains four extracellular immunoglobinlike domains. The TCR, in association with CD4, can bind class II MHC molecules presented by the antigen-presenting cells. The CD4 protein functions by increasing the avidity of the interaction between the TCR and an antigen-class II MHC complex.
Synonyms	CD8 alpha chain, CD8A, MAL
Reference Data

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