GNRHR Mouse Monoclonal Antibody [Clone ID: F1G4]

Specifications

Product Data
Clone Name	F1G4
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA. Flow Cytometry. Western Blot: 1-2 µg/ml Immunohistochemistry on Frozen Sections: The Monoclonal antibody F1G4 specifically stains Human GnRH receptors on frozen tissue of the anterior pituitary.   The mAb has been found to react with T47D breast carcinoma cells.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Mice were immunized with a BSA-conjugated peptide corresponding to amino acids 1-29 of Human GnRH receptor extracellular domain. Splenocytes were fused with SP2/0 mouse myeloma cells. Stability was established by subcloning four times.
Specificity	This Monoclonal antibody F1G4 reacts with GnRH receptors in the anterior pituitary.  F1G4 showed specificity for the synthetic peptide in ELISA and Dot blot.
Formulation	PBS State: Purified State: Liquid purified Ig fraction Preservative: 0.05% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or  (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	gonadotropin releasing hormone receptor
Database Link	Entrez Gene 2798 Human UniProt ID P30968
Background	Gonadotropin Releasing Hormone (GnRH) is down-regulated by hCG and believed to be an autocrine factor that regulates the ovary. The Gonadotropin Releasing Hormone Receptor (GnRHR) is synthesized in the pituitary gland. GnRH stimulates the gonadotrophs of the anterior pituitary to secrete luteinising hormone (LH) as well as follicle-stimulating hormone (FSH). The receptor contains of seven hydrophobic transmembrane domains connected by hydrophillic extracellular, and intracellular loops characteristic of G-protein coupled receptors.
Synonyms	Gonadotropin-releasing hormone receptor, GnRH-R, GRHR
Reference Data

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