MRP1 (ABCC1) Mouse Monoclonal Antibody [Clone ID: MRPm6]

Specifications

Product Data
Clone Name	MRPm6
Applications	IF, IHC, WB
Recommend Dilution	Flow Cytometry: 1/20-1/50.  Cell permeabilization required with 10% lyzing solution followed by primairy antibody and anti-Mouse-FITC.  Western blotting: 1/20-1/50. Immunocytochemistry on Aceton-Fixed Cell Preparation: 1/20-1/50. Immunohistochemstry on Acetone-Fixed Frozen Sections: 1/20-1/50, Immunocytochemistry on Formalin-Fixed Paraffin Embedded Tissues 1/20.  Microwave pretreatment (0.01M Citrate Buffer,10min at 100°C) may increase the performance of this antibody.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Bacterial fusion protein of MRP, containing a segment of 170 amino acids in the carboxy terminal end and part of the carboxy proximal nucleotide binding domain of the protein.
Specificity	This Monoclonal antibody MRPm6 reacts with an internal epitope of MRP, a 180-195 kD transmembrane transporter protein overexpressed in various Human non-P-glycoprotein MDR tumor cell lines.  MRPm6 does not cross react with the Human MDR1 and MDR3 gene products (Flens et al. 1994).
Formulation	State: Supernatant State: Serum-Free Tissue Culture Supernatant Stabilizer: 0.7% BSA Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	ATP binding cassette subfamily C member 1
Database Link	Entrez Gene 4363 Human UniProt ID P33527
Background	MRP1 (also known as ABCC, GS-X, ABC29) functions as a multispecific organic anion transporter, with (oxidized) glutathione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. It also transports drugs and other hydrophobic compounds in presence of glutathione. MRP1 protein is found predominantly in the heart, lung, small intestine, brain, and skin.
Synonyms	Multidrug resistance-associated protein 1, LTC4 transporter
Note	Mab producing cells: The hybridoma cell line was obtained by fusion of lymph node cells from an immunized mouse (Balb/C) with SP2/0 mouse myeloma cells. Culture Medium: RPMI-1640 (Gibco, Paisley, Scotland UK), supplemented with Nutridoma-SR (Boehringer, Indianapolis, USA). The medium does not contain serum nor added enzymes. The antibody solution has been filtered through a 0.22 micron filter. NOTE: This monoclonal antibody has been produced in a clinical laboratory in which no animal viruses are being studied or cultured.
Reference Data

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