MRP3 (ABCC3) Mouse Monoclonal Antibody [Clone ID: M3II-9]

Specifications

Product Data
Clone Name	M3II-9
Applications	IF, IHC, WB
Recommend Dilution	Western blotting: Use 1/20-1/50 dilution and anti Mouse HRP  Immunocytochemistry: Use 1/20-1/50 on acetone or formaldehyde fixed cytospin preparations.  Immunohistochemistry on Acetone Fixed Frozen Sections: 1/20. This Monoclonal antibody M3II-9 has potential value for detection of MRP3-mediated drug-resistance in Human tumor samples.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Bacterial fusion protein of MRP3, containing amino acids 830-949 of the protein.
Specificity	This Monoclonal antibody M3II-9 reacts with an internal epitope of MRP3, a 190-200 kD transmembrane protein that is closely related to the multidrug resistance protein MRP1.  It does not cross-react with the Human MDR1, MRP1, MRP2 or MRP5 gene products.
Formulation	State: Supernatant State: Serum Free Tissue Culture Supernatant Stabilizer: 0.7% BSA Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	ATP binding cassette subfamily C member 3
Database Link	Entrez Gene 8714 Human UniProt ID O15438
Background	Human multidrug-resistance protein (MRP) gene family is composed of multiple members including MRP 1, MRP 2 or cMOAT and four other homologous genes called MRP 3, MRP 4, MRP 5 and MRP 6. Canalicular Multi Organic Anion Transporter 2 / Multidrug Resistance Associated Protein 3 (cMOAT 2 / MRP 3) has a molecular weight of 190-200 kD. MRP3 is closest to MRP1 with 58% amino acid identity. MRP 3 has been shown to be mainly expressed in the liver, colon, intestine, adrenal gland and, to a lower extent, in several other tissues.
Synonyms	Multidrug resistance-associated protein 3, MLP2
Note	Mab producing cells: The hybridoma cell line was obtained by fusion of lymph node cells from an immunized mouse (Balb/C) with SP2/0 mouse myeloma cells. Culture Medium: RPMI-1640 (Gibco, Paisley, Scotland UK), supplemented with Nutridoma-SR (Boehringer, Indianapolis, USA). The medium does not contain serum nor added enzymes. The antibody solution has been filtered through a 0.22 micron filter. NOTE: This monoclonal antibody has been produced in a clinical laboratory in which no animal viruses are being studied or cultured.
Reference Data

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